Determinación de nuevos biomarcadores mitocondriales en el desarrollo de la miocardiopatía isquémica mediante aplicaciones genómicas y proteómicas.

OBJETIVES: The study of the role of mitochondria in cardiomyopathies is of greatest interest because may help to clarify the pathogenic mechanisms that initiate a disease of such importance in the Western world health as heart failure and may open new ways of enabling research on risk factors and identification of therapeutic targets for this disease. Therefore, we propose the following aims: 1) Study the distribution of mitochondrial DNA haplogroups as a risk factor in the development of heart failure in subjects with cardiomyopathies of different índole.2) Compare the proteomes of healthy hearts and hearts with ischemic to try to identify possible mitochondrial protein involved in the disease process occurred. METHODOLOGY – Identification of haplogroups by Snapshot and sequencing of fragments of mitochondrial DNA in blood samples of patients with heart failure. – A sequential proteomic study of proteins involved in the development of ischemic DIGE analysis by 2D-PAGE – Experimental porcine model of myocardial infarction. – Analysis of the tissue expression of proteins by Inmunoblotting and / or Immunohistochemistry, and studies of gene expression by qRT-PCR.